Nonrigid registration of three-dimensional ultrasound and magnetic resonance images of the carotid arteries

Atherosclerosis at the carotid bifurcation can result in cerebral emboli, which in turn can block the blood supply to the brain causing ischemic strokes. Noninvasive imaging tools that better characterize arterial wall, and atherosclerotic plaque structure and composition may help to determine the factors which lead to the development of unstable lesions, and identify patients at risk of plaque disruption and stroke. Carotid magnetic resonance (MR) imaging allows for the characterization of carotid vessel wall and plaque composition, the characterization of normal and pathological arterial wall, the quantification of plaque size, and the detection of plaque integrity. On the other hand, various ultrasound (US) measurements have also been used to quantify atherosclerosis, carotid stenosis, intima-media thickness, total plaque volume, total plaque area, and vessel wall volume. Combining the complementary information provided by 3D MR and US carotid images may lead to a better understanding of the underlying compositional and textural factors that define plaque and wall vulnerability, which may lead to better and more effective stroke prevention strategies and patient management. Combining these images requires nonrigid registration to correct the nonlinear misalignments caused by relative twisting and bending in the neck due to different head positions during the two image acquisition sessions. The high degree of freedom and large number of parameters associated with existing nonrigid image registration methods causes several problems including unnatural plaque morphology alteration, high computational complexity, and low reliability. Thus, a twisting and bending model was used with only six parameters to model the normal movement of the neck for nonrigid registration. The registration technique was evaluated using 3D US and MR carotid images at two field strengths, 1.5 and 3.0 T, of the same subject acquired on the same day. The mean registration error between the segmented carotid artery wall boundaries in the target US image and the registered MR images was calculated using a distance-based error metric after applying a twisting and bending model based nonrigid registration algorithm. An average registration error of 1.4 +/- 0.3 mm was obtained for 1.5 T MR and 1.5 +/- 0.4 mm for 3.0 T MR, when registered with 3D US images using the nonrigid registration technique presented in this paper. Visual inspection of segmented vessel surfaces also showed a substantial improvement of alignment with this nonrigid registration technique compared to rigid registration

The spiritual experiences of students of Iran University of Medical Sciences in 2020

Background: Psychologists consider the acceptance of spirituality as a cultural reality and by acknowledging the positive effects of spirituality on mental health, the World Health Organization also considers the spiritual dimension as the physical, social and emotional dimensions of human existence. Spirituality is a genuine and inner experience that It lies in the nature of every human being. The purpose of this study was to investigate the spiritual experiences of students of Iran University of Medical Sciences in 2020. Methods: The present study was descriptive-analytical. The sample size of 500 students of Iran University of Medical Sciences in 2020 was estimated. For sampling, a list of students was prepared from the faculty education and the sample was selected by regular random sampling method through random number generation software. The data collection tool was a questionnaire with demographic questions and spiritual experiences. The collected data were analyzed after completion with SPSS 20 software. A significance level of 0.05 was considered. Results: The mean score of the meaning-finding component in life was obtained at 57.98. There was a significant relationship between spiritual experiences and gender, age and educational level of students, while no significant relationship was found between spiritual experiences and marital status and residence. Conclusion: In order to ensure the spiritual health of students, it is necessary to plan properly to create a meaningful atmosphere in universities for different age and gender groups

Scrutinizing relationships between submarine groundwater discharge and upstream areas using thermal remote sensing: A case study in the northern Persian gulf

© 2021 by the authors. Licensee MDPI, Basel, Switzerland. Nutrient input through submarine groundwater discharge (SGD) often plays a significant role in primary productivity and nutrient cycling in the coastal areas. Understanding relationships between SGD and topo-hydrological and geo-environmental characteristics of upstream zones is essential for sustainable development in these areas. However, these important relationships have not yet been completely explored using data-mining approaches, especially in arid and semi-arid coastal lands. Here, Landsat 8 thermal sensor data were used to identify potential sites of SGD at a regional scale. Relationships between the remotely-sensed sea surface temperature (SST) patterns and geoenvironmental variables of upland watersheds were analyzed using logistic regression model for the first time. The accuracy of the predictions was evaluated using the area under the receiver operating characteristic curve (AUC-ROC) metric. A highly accurate model, with the AUC-ROC of 96.6%, was generated. Moreover, the results indicated that the percentage of karstic lithological formation and topographic wetness index were key variables influencing SGD phenomenon and spatial distribution in the northern coastal areas of the Persian Gulf. The adopted methodology and applied metrics can be transferred to other coastal regions as a rapid assessment procedure for SGD site detection. Moreover, the results can help planners and decision-makers to develop efficient environmental management strategies and the design of comprehensive sustainable development policies

Lack of association of two common polymorphisms on 9p21 with risk of coronary heart disease and myocardial infarction; results from a prospective cohort study

Background: Recent genome wide association (GWA) studies identified two Single Nucleotide Polymorphisms (SNP) (rs10757278 and rs10757274) in the region of the CDK2NA and CDK2NB genes to be consistently associated with the risks of coronary heart disease (CHD) and myocardial infarction (MI). We examined the SNPs in relation to the risk of CHD and MI in a large population based study of elderly population. Methods: The Rotterdam Study is a population-based, prospective cohort study among 7983 participants aged 55 years and older. Associations of the polymorphisms with CHD and MI were assessed by use of Cox proportional hazards analyses. Results: In an additive model, the age and sex adjusted hazard ratios (HRs) (95% confidence interval) for CHD and MI were 1.03 (0.90, 1.18) and 0.94 (0.82, 1.08) per copy of the G allele of rs10757274. The corresponding HRs were 1.03 (0.90, 1.18) and 0.93 (0.81, 1.06) for the G allele of rs10757278. The association of the SNPs with CHD and MI was not significant in any of the subgroups of CHD risk factors. Conclusion: We were not able to show an association of the studied SNPs with risks of CHD and MI. This may be due to differences in genes involved in the occurrence of CHD in young and older people

Determination of suitable feeds for western white shrimp (Litopenaeus vannamei) broodstock maturation

For the successful breeding of western white shrimps (Litopenaeus vannamei) brooders (females and males), we used cuttle fish (Sepia pharaonis) and sand worm (Perinereis nuntica) of Persian Gulf as principle feeds, 3 times a day, and from cow livier and edible bivalve meat (Solen brevis) of Persian Gulf, as secondary feeds, 1 time a day. Nutritional value of Sepia pharaonis, Perinereis nuntica and Solen brevis, with determination of the amount of fatty acids such as arachidonic acid (20:4n-6), stearic acid (18:00), oleic acid (18:1n-9), palmitic acid, palmitoleic acid (16:1n-7), eicosapentaenoic acid (20:5n-3) and docosahexaenoic acid, essential amino acids inclusive arginine, lysine, leucine, isoleusine, threonine, valine, histidine, phenylalanine, methionine, tryptophane and crude protein, crude fat, crude fiber, ash and moisture were done in laboratory. The results showed that, the amount of essential amino acides and nonessential amino acides (in the more causes) in Sepia pharaonis were more than Solen brevis and in Solen brevis more than Perinereis nuntica. Only nonessential amino acides, glysine and alanine in Solen brevis were more than Sepia pharaonis. Also in chemical analysis of wet feeds determined that, crude protein percent in cattle fish was 2.9 times and 2.2 times more than Solen brevis and Perinereis nuntica, respectively. The crude fat in cattle fish was 2.1 times and 1.6 times more than Solen brevis and Perinereis nuntica respectively. Tottaly, the amount of essential fatty acides in Sepia pharaonis and Perinereis nuntica were more than Solen brevis, and in Sepia pharaonis and Perinereis nuntica were almost, equal. Total lipids of Sepia pharaonis, Perinereis nuntica and Solen brevis determined 7.92, 5.90 and 3.63 percent respectively. Totally the percent of fatty acids in cattle fish and Perinereis nuntica is almost equal and was more than Solen brevis meat. We can noted that, the Sepia pharaonis and Perinereis nuntica are suitable as principle feed and Solen brevis and cow livier, as secondary feed for L.vannamei broodstock maturation

Cardiac MR Elastography: Comparison with left ventricular pressure measurement

Purpose of the Study: To compare magnetic resonance elastography (MRE) with ventricular pressure changes in an animal model. Methods: Three pigs of different cardiac physiology (weight, 25 to 53 kg; heart rate, 61 to 93 bpm; left ventricular [LV] end-diastolic volume, 35 to 70 ml) were subjected to invasive LV pressure measurement by catheter and noninvasive cardiac MRE. Cardiac MRE was performed in a short-axis view of the heart and applying a 48.3-Hz shear-wave stimulus. Relative changes in LV-shear wave amplitudes during the cardiac cycle were analyzed. Correlation coefficients between wave amplitudes and LV pressure as well as between wave amplitudes and LV diameter were determined. Results: A relationship between MRE and LV pressure was observed in all three animals (R-square [greater than or equal to] 0.76). No correlation was observed between MRE and LV diameter (R-square [less than or equal to] 0.15). Instead, shear wave amplitudes decreased 102 +/- 58 ms earlier than LV diameters at systole and amplitudes increased 175 +/- 40 ms before LV dilatation at diastole. Amplitude ratios between diastole and systole ranged from 2.0 to 2.8, corresponding to LV pressure differences of 60 to 73 mmHg. Conclusion: Externally induced shear waves provide information reflecting intraventricular pressure changes which, if substantiated in further experiments, has potential to make cardiac MRE a unique noninvasive imaging modality for measuring pressure-volume function of the heart

RANTES/CCL5 and risk for coronary events:Results from the MONICA/KORA Augsburg case-cohort, Athero-express and CARDIoGRAM studies

Background: The chemokine RANTES (regulated on activation, normal T-cell expressed and secreted)/CCL5 is involved in the pathogenesis of cardiovascular disease in mice, whereas less is known in humans. We hypothesised that its relevance for atherosclerosis should be reflected by associations between CCL5 gene variants, RANTES serum concentrations and protein levels in atherosclerotic plaques and risk for coronary events. Methods and Findings: We conducted a case-cohort study within the population-based MONICA/KORA Augsburg studies. Baseline RANTES serum levels were measured in 363 individuals with incident coronary events and 1,908 non-cases (mean follow-up: 10.2±4.8 years). Cox proportional hazard models adjusting for age, sex, body mass index, metabolic factors and lifestyle factors revealed no significant association between RANTES and incident coronary events (HR [95% CI] for increasing RANTES tertiles 1.0, 1.03 [0.75-1.42] and 1.11 [0.81-1.54]). None of six CCL5 single nucleotide polymorphisms and no common haplotype showed significant associations with coronary events. Also in the CARDIoGRAM study (&gt;22,000 cases, &gt;60,000 controls), none of these CCL5 SNPs was significantly associated with coronary artery disease. In the prospective Athero-Express biobank study, RANTES plaque levels were measured in 606 atherosclerotic lesions from patients who underwent carotid endarterectomy. RANTES content in atherosclerotic plaques was positively associated with macrophage infiltration and inversely associated with plaque calcification. However, there was no significant association between RANTES content in plaques and risk for coronary events (mean follow-up 2.8±0.8 years). Conclusions: High RANTES plaque levels were associated with an unstable plaque phenotype. However, the absence of associations between (i) RANTES serum levels, (ii) CCL5 genotypes and (iii) RANTES content in carotid plaques and either coronary artery disease or incident coronary events in our cohorts suggests that RANTES may not be a novel coronary risk biomarker. However, the potential relevance of RANTES levels in platelet-poor plasma needs to be investigated in further studies.</p

Genomic correlates of glatiramer acetate adverse cardiovascular effects lead to a novel locus mediating coronary risk

Glatiramer acetate is used therapeutically in multiple sclerosis but also known for adverse effects including elevated coronary artery disease (CAD) risk. The mechanisms underlying the cardiovascular side effects of the medication are unclear. Here, we made use of the chromosomal variation in the genes that are known to be affected by glatiramer treatment. Focusing on genes and gene products reported by drug-gene interaction database to interact with glatiramer acetate we explored a large meta-analysis on CAD genome-wide association studies aiming firstly, to investigate whether variants in these genes also affect cardiovascular risk and secondly, to identify new CAD risk genes. We traced association signals in a 200-kb region around genomic positions of genes interacting with glatiramer in up to 60 801 CAD cases and 123 504 controls. We validated the identified association in additional 21 934 CAD cases and 76 087 controls. We identified three new CAD risk alleles within the TGFB1 region on chromosome 19 that independently affect CAD risk. The lead SNP rs12459996 was genome-wide significantly associated with CAD in the extended meta-analysis (odds ratio 1.09, p = 1.58×10-12). The other two SNPs at the locus were not in linkage disequilibrium with the lead SNP and by a conditional analysis showed p-values of 4.05 × 10-10 and 2.21 × 10-6. Thus, studying genes reported to interact with glatiramer acetate we identified genetic variants that concordantly with the drug increase the risk of CAD. Of these, TGFB1 displayed signal for association. Indeed, the gene has been associated with CAD previously in both in vivo and in vitro studies. Here we establish genome-wide significant association with CAD in large human samples.This work was supported by grants from the Fondation Leducq (CADgenomics: Understanding CAD Genes, 12CVD02), the German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (e:AtheroSysMed, grant 01ZX1313A-2014 and SysInflame, grant 01ZX1306A), and the European Union Seventh Framework Programme FP7/2007-2013 under grant agreement no HEALTH-F2-2013-601456 (CVgenes-at-target). Further grants were received from the DFG as part of the Sonderforschungsbereich CRC 1123 (B2). T.K. was supported by a DZHK Rotation Grant. I.B. was supported by the Deutsche Forschungsgemeinschaft (DFG) cluster of excellence ‘Inflammation at Interfaces’. F.W.A. is supported by a Dekker scholarship-Junior Staff Member 2014T001 - Netherlands Heart Foundation and UCL Hospitals NIHR Biomedical Research Centre